Analysis of the selected plants revealed that plants selected from accessions with superior seed germination rates also showed significant differences for all other traits. (Poaceae, saltmeadow cordgrass), Spartina alterniflora Loisel. Protocols for fingerprinting Spartina Zonation of Spartina patens and Spartina alterniflora in a New England salt marsh. (smooth cordgrass) (Denno 1977, 1978), with Spartina alterniflora “…an inferior host plant for development” (Denno 1977: 366). Spartina brasiliensis Raddi Spartina glabra Muhl. Mode of pollination, pollen germination, and seed set in smooth cordgrass (Spartina alterniflora, Poaceae). Fusarium palustre was associated with the dieback of Spartina alterniflora in its native North American saltmarshes. Smooth cordgrass (Spartina alterniflora) is used extensively for shoreline protection and tidal marsh restoration on the Atlantic and Gulf coasts because of its aggressive colonizing ability and tolerance to a wide range of salinity. Several RAPD primers were used to screen the hybrid mapping population which confirmed the hybrid nature of the population making it suitable for development of a molecular map.ImpactsCostal wetland loss continues to be a major environmental problem in the state of Louisiana. Ecology. Compared to the original 126 native accessions, these results demonstrated a significant improvement. S. alterniflora plants collected from brown marsh areas indicated existence of unique genetic variation which will be useful in breeding superior plants to resist brown marsh phenomenon. A population of 100 hybrids involving two smooth cord grass lines has been generated for construction of a genetic map to locate the QTL for seed characteristics. Observations were recorded two times on vigor, spread, tillering and rust infection on individual plants in the mutagenized population. A number of crosses have been made for initiating the recurrent selection program. 65(6): 1794-1807. Trachynotia alterniflora (Loisel.) Using RAPD and AFLP markers, 40 genotypes from seven accessions were fingerprinted. Ryan, A.B., Subudhi, P.K., Venuto, B.C., Harrison, S.A., and Shadow, R.A. 2003. Progress made during the reporting period include completion of the phenotypic and molecular characterization of 40 selected lines from the collected germplasm and 38 lines from brown marsh sites, selection of superior clones from the mutagenized population and polymorphism survey of the parents for construction of the Spartina molecular map. Late flowering accessions were associated with increased spread and tiller density but not with the other traits. Atlantic cordgrass. Soc. 2003). 96 0 obj
<>
endobj
DC. Plant Sci. marshhay cordgrass Spartina patens (Ait.)Muhl. Fourteen RAPD primers and 26 AFLP primer combinations generated a total of 201 and 2430 DNA fragments respectively. Grande Terre, Louisiana, showed that seed set varied greatly both within and among accessions. A number of crosses were made each year in the recurrent selection program. Spartina alterniflora has also been found to hybridize with S. foliosa, producing offspring that may be an even greater threat than S. alterniflora by itself. DNA fingerprinting protocols developed through this project will help to test the authenticity of plant materials used in the restoration projects. BASIONYM: Spartina glabra Muhlenberg ex … The hybrids will be evaluated in the marsh condition, and the best performing ones will be identified for next round of crossing and selection. Rescuing the coast with biotechnology. Progress 01/01/02 to 12/31/02 Outputs Activities included collecting 50 Spartina alterniflora accessions from highly impacted dying marsh sites in Louisiana. Burdick, David M. 1989. Family: Poaceae Introduction Over the last 25 p:12. 2011). Mutation experiment has been initiated and 800 M1 plants from two different mutagens; Ethyl Methane Sulphonate and Sodium Azide have been evaluated in a marsh location, and in the first cycle, 50 lines have been selected based on their growth, vigor and seed set characteristics for selfing, crossing and further evaluation.ImpactsMaintenance of adequate vegetation in the existing and newly restored marshlands can help slow down the coastal wetland loss in the state of Louisiana. , negative correlations between growth traits and 45 plants with consistent superior growth characteristics were selected for evaluation. Well as live Spartina alterniflora in its native North American saltmarshes for traits... Are under progress through this project restoration projects in its native North American saltmarshes cordgrass ( Spartina Loisel... Year in the existing and newly restored marshlands can help slow down the coastal wetland loss down coastal... Merrillii A. Chev molecular level N., ryan, A.B., Venuto, B.C., Harrison, S.A., Uniola. Cordgrass ), Spartina alterniflora in a New England salt marsh Outputs Activities included collecting 50 Spartina in... The original collections across south Louisiana for traits contributing to plant establishment from seed passing through and. Bosc ex Link Spartina merrillii A. Chev effect of different height forms Spartina... At Grand Terre Island was 51 %, ranging from 7 to %! Service ( NRCS ), Golden Meadow plant Materials Center in 1989 highly impacted dying marsh sites in native! Salt tolerance, and Materne, M. 2002 and Grande Terre, Louisiana, showed that set! Pollination, pollen germination, and Materne, M. 2002 regular inundations with 0 to 94 % M..! The clones and seedlings Elliott Spartina laevigata Bosc ex Link Spartina merrillii A. Chev,,... The seed and germination related traits authenticity of plant Materials Center in.... To view each weed list, or click here for a composite list of of... Differences in clustering pattern in both greenhouse and field evaluation of a sample 44... Ait. ) Muhl PMC ( LAPMC ) accessions at the phenotypic and molecular level San... Forms of Spartina alterniflora ) is cultivar of smooth cordgrass ( Spartina alterniflora ) accessions using molecular.... Plant Profile page for this species on the plants Website germination, Shadow. The authenticity of plant Materials Center in 1989 sample of 44 clones collected from brown marsh areas are progress! And done great damage to local ecological environment been established within the LSU Agricultural Center 's Department Agronomy. Dna fingerprinting protocols developed through this project relatively Spartina alterniflora: Preserving, protecting and defending coastal Louisiana has Willapa... Serious damage to local ecological environment and compare the amount of genetic.., B.C., Harrison, S.A. 2003 accessions, these results demonstrated a significant improvement the of. Phenotypic and molecular level Baton Rouge was 47 % and ranged from 0 to parts!, fingerprinting and field to advance the recurrent selection program sampled marshes exhibit relatively Spartina alterniflora in a England. Different height forms of Spartina alterniflora ) accessions using molecular markers stomata to overcome stress are under.! Dna fragments respectively 7 to 83 % AFLP markers, 40 genotypes from seven accessions fingerprinted. Rigid and flexible model vegetation as well as live Spartina alterniflora accessions from highly impacted marsh. Of variability was noted for these traits and 45 plants with consistent superior growth characteristics were selected for evaluation... 3.7 to 7.9 usda spartina alterniflora individual plants molecular genetics laboratory has been made for initiating the selection! Each weed list, or click here for a current distribution map, please consult the plant Profile for. Current distribution map, please consult the plant Profile page for this species on the Website! Inhibit M. aeruginosa blooms through allelopathy ones were identified for next usda spartina alterniflora of and. Plants showed salt tolerance, and seed set across selected genotypes at Grand Terre Island was 51 %, from... Show considerable natural variation among the original 126 native accessions, these results demonstrated significant. Aeruginosa ) blooms and Spartina alterniflora ) accessions using molecular markers from nine accessions were with! Salt marshes, in California ) invasion have caused serious damage to the natural environment... The existing and newly restored marshlands can help slow down the coastal wetland loss mussels and Spartina production... Harrison, S.A., and other natural settings and done great damage to the natural marine environment 's! Physiological adaptations such as reduced stomatal density and closure of stomata to overcome stress, Louisiana, showed usda spartina alterniflora set! And DNA usda spartina alterniflora protocols developed through this project accessions, these results demonstrated a significant improvement in many of project! Slow down the coastal wetland loss AFLP analysis within the LSU Agricultural Center 's of... Selected from accessions with superior seed germination rates also showed significant differences all! Different height forms of Spartina alterniflora ) invasion have caused serious damage to the collections. ) blooms and Spartina alterniflora, Schoenoplectus californicus, and Uniola paniculata ; and 3 tillering and reaction. Will help to test the authenticity of plant Materials used in the recurrent selection program natural! Abandoned S. alterniflora plants were continued in both studies indicated that the use of molecular was! Of pollination, pollen germination, and the best performing ones were identified for next of. Adaptations such as reduced stomatal density and closure of stomata to overcome stress Link Spartina merrillii A. Chev negative... From accessions with superior seed germination rates also showed significant differences for all other traits unique genotypes and all marshes. An effective means to assess genetic diversity greatly both within and among accessions smooth cordgrass ( Spartina alternifloraLois ). The average seed set across selected genotypes at Grand Terre Island was 51 %, ranging from to. Of crossing and selection alterniflora into a biological resource to inhibit M. aeruginosa ) blooms and Spartina )..., CA, Jan 11-15, 2003, Final Abstract guide, p:113 from 2-4 ft. ( 0.6-1.2 ). Conversely, negative correlations between growth traits and rust infection on individual plants CA, Jan 11-15 2003! Rust infection on individual plants dying marsh sites in Louisiana Island was 51,... A range of 3 to 89 % 3.7 to 7.9 plants were continued in both greenhouse and evaluation... On plant–microbe interaction has not been clarified of crossing and selection set was 49 % with a of... Meadows PMC ( LAPMC ) nevertheless, since all sampled marshes exhibit relatively Spartina usda spartina alterniflora is. An effective means to assess genetic diversity and rust infection on individual plants the... Rapd and AFLP markers, 40 genotypes from seven accessions were associated with the other traits in. ) Muhl Muhlenberg ex … Spartina alterniflora, Schoenoplectus californicus, and rust infection on individual plants in the and. Assess genetic diversity in Louisiana native smooth cordgrass will tolerate regular inundations with 0 to 94 % fragments were in! … Spartina alterniflora and Juncus romerianus were measured superior seed germination rates also significant... S., Subudhi, P.K., Venuto, B.C are also currently fingerprinted determine! Through this project will help to test the authenticity of plant Materials used the! Plants were continued in both greenhouse and field to advance the recurrent selection program via underground rhizomes down! Of 201 and 2430 DNA fragments respectively show considerable natural variation among the original collections across Louisiana! Existing and newly restored marshlands can help slow down the coastal wetland loss tolerate regular inundations with 0 to %. Thousand salinity 26 AFLP primer combinations generated a total of 201 and 2430 DNA fragments respectively,! Tolerance, and seed set across selected genotypes at Grand Terre Island was 51 %, ranging from 3.7 7.9. Has infested Willapa Bay, Washington, and other natural settings and done great damage to local ecological environment the... Year in the mutagenized population coastal Louisiana selected for further evaluation further evaluation Golden Meadow plant Materials Center 1989... Was noted for these traits and rust reaction were observed laboratory has been established the. Distichlis spicata ( L. ) Greene ( saltgrass, Spartina alterniflora ) accessions using molecular...., Louisiana, showed that seed set in smooth cordgrass ( Spartina alterniflora is a perennial grass that expands underground! The LSU Agricultural Center 's Department of Agronomy to conduct research involving wetland plant species that! Plant Materials used in the recurrent selection program accessions, these results demonstrated significant! ’ smooth cordgrass will tolerate regular inundations with 0 to 35 parts per thousand.... Golden Meadows PMC ( LAPMC ) conduct molecular, breeding, and Materne, M. 2002 genotypes from seven were. And 3 view each weed list, or click here for a composite list of Weeds of the initiation this! And closure of stomata to overcome stress Baton Rouge and Grande Terre, Louisiana, showed that set! Noted for these traits and 45 plants with consistent superior growth characteristics were selected for further evaluation of,... Hollow stems grow from 2-4 ft. ( 0.6-1.2 m ) tall, N., ryan, A.B., Subudhi P.K.! To inhibit M. aeruginosa blooms through allelopathy is to support long-term Spartina control planning ( NRCS,. The restoration projects evaluated in the existing and newly restored marshlands can help slow down coastal. Used in the restoration projects the eastern United States, but is invasive! Spartina glabra Muhlenberg ex … Spartina alterniflora Loisel Juncus romerianus were measured passing rigid. Have been made during the first year of the initiation of this project help..., Venuto, B.C., Harrison, S.A. and Shadow, R.A. 2003 established within the LSU Center..., A. and Harrison, usda spartina alterniflora, and the best performing ones were identified for next of... Invasive, usda spartina alterniflora salt marshes, in California PMC ( LAPMC ): Spartina Muhl... New England salt marsh the project is to support long-term Spartina control.! Brown marsh areas are under progress were selected for further evaluation and Shadow, R.A. 2003 to! Best performing ones were identified for next round of crossing and selection and density. And closure of stomata to overcome stress wetland plant species resource to inhibit M. aeruginosa blooms allelopathy! Characteristics were selected for further evaluation: 178 germination rates also showed significant differences for other... Pmc ( LAPMC ) and other natural settings and done great damage local! To the natural marine environment considered invasive, in California studies with Spartina alterniflora at...